cyanobase / GCA_000153285.1 - WH7805

BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase (462 letters) Database: GCA_000317125.1P 5752 sequences; 1,839,647 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Chro_4920 UDP-N-acetylmuramoylalanine--D-glutamate ligase 353 e-117 Chro_2645 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine l... 55 4e-09 Chro_1866 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopi... 44 3e-05 Chro_1549 UDP-N-acetylmuramate--L-alanine ligase 39 6e-04 Chro_5104 protein of unknown function DUF1727 39 8e-04 Chro_5574 amine oxidase 32 0.10 Chro_2386 ABC-2 type transporter 31 0.16 Chro_5111 C-3',4' desaturase CrtD 32 0.19 Chro_0203 short-chain dehydrogenase/reductase SDR 30 0.57 Chro_2389 amine oxidase 30 0.60 1e5d9b4b725dcc236130320c94b9e4c6

>Chro_4920 UDP-N-acetylmuramoylalanine--D-glutamate ligase Length = 464 Score = 353 bits (907), Expect = e-117, Method: Compositional matrix adjust. Identities = 208/465 (44%), Positives = 278/465 (59%), Gaps = 17/465 (3%) Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLEL 60 MS + V+GLG+SG AARLL+ +G+ V++ +R D +LR + L + I ++LG L Sbjct: 1 MSNACVIGLGKSGVAAARLLKREGWQVILSDRSDSASLRQQQQQLAAEGIPVRLGHTPSL 60 Query: 61 DSFTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNG 120 DS DS +V+SPG+ WD L+ RA G GEM +AW L +PW+GITGTNG Sbjct: 61 DS----TDSPQLIVVSPGVPWDADILNRARALGIETIGEMELAWRYLNAVPWVGITGTNG 116 Query: 121 KTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTR--------PKPDWVVMEMS 172 KTT T L+ + AGL AP GN+G++ ++ALE +R PDW++ E+S Sbjct: 117 KTTTTALVAAIFQTAGLNAPACGNIGHAVCDVALEFQNNSRGAQPYAPTKYPDWIIAEIS 176 Query: 173 SYQIEAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQH 232 SYQIE+A V+PRIG+WTT TPDHL RH T+E Y IK L+ +S++ V+N DDA + Q Sbjct: 177 SYQIESAPSVAPRIGVWTTFTPDHLSRHKTLERYYDIKAHLVHQSQLQVINGDDAFLSQE 236 Query: 233 -CGHWSRSCIRWVSCGDHSPEGGNPQL-TVNAQGWVSRGEQHLFPADSLPLPGEHNRQNM 290 W + W S + +P L T WV G++ + +L + G HN QN+ Sbjct: 237 KLNDWQDA--YWTSVKGKAFLNCSPDLGTYIEDDWVIFGQEQIVQVSALRMVGAHNLQNL 294 Query: 291 LLVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKATNYDAAAVGLRS 350 L+ A A AG+ I A+ F GVPHRLE + + GI NDSKATNYDAA VGL S Sbjct: 295 LMAVAVARLAGIDKEAIATAIATFPGVPHRLEHICTWQGIDFINDSKATNYDAAQVGLSS 354 Query: 351 VQAPVVLLAGGQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLIETSGFAGPMIHCAD 410 V +P +L+AGG+ K GD + WL+ IQ+Q A V+L G+ A A + G+ I Sbjct: 355 VTSPAILIAGGEAKIGDDLAWLQTIQAQAAAVLLIGDAAPAFASRLSQVGYNNYEI-VET 413 Query: 411 LDSAVSQALSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQL 455 + AVS+A LA QA VLLSPACASFD+Y NFE RGD FRQL Sbjct: 414 MARAVSRAAELAPQYQARVVLLSPACASFDRYQNFEQRGDDFRQL 458 >Chro_2645 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase Length = 452 Score = 55.5 bits (132), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 65/227 (28%), Positives = 97/227 (42%), Gaps = 42/227 (18%) Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEV-----LQ 158 W + +P IG+TG+ GKTT L+ VL GNV + A E+ L Sbjct: 100 WRSQFHMPVIGVTGSVGKTTTKELIAAVLG-------TQGNVLKTHANFNNEIGVPKTLL 152 Query: 159 GTRPKPDWVVMEMSSY---QIEAAAEVS-PRIGIWTTLTPDHLERHGTIEAYRAIKRGLL 214 G + D+ V+EM+ +I +++ P IG+ T + H+ER G+ EA K LL Sbjct: 153 GLDTQHDFAVIEMAMRGRGEIALLTQIARPTIGVITNVGTAHIERLGSEEAIAQAKCELL 212 Query: 215 E---RSEIPVLNADDADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVSRGE- 270 ++ + +LN D+ R +S + +G +T +G RGE Sbjct: 213 AEMPQNSVAILNYDN--------------FRLISTANTVWQG--KTITYGLEGGDIRGEL 256 Query: 271 ---QHLFPAD---SLPLPGEHNRQNMLLVTAAALAAGVSASTIEAAL 311 Q L A LPLPG HN N L A A + S ++ L Sbjct: 257 LDSQTLKVAGLELPLPLPGRHNAVNFLAALAVAKVLQIDWSVLKTGL 303 >Chro_1866 UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase Length = 510 Score = 43.5 bits (101), Expect = 3e-05, Method: Compositional matrix adjust. Identities = 59/226 (26%), Positives = 92/226 (40%), Gaps = 38/226 (16%) Query: 126 HLLQHVLAHAGLEAPMAGNVGYS---------------AAELALEVLQGTRPKPDWVVME 170 HL++ L A L + G + YS A EL ++ + VME Sbjct: 130 HLIEFFLQQAKLSTALFGTL-YSRWAGFEQTAAHTTPFAVELQQQLANAVQAGTQVAVME 188 Query: 171 MSSYQIEAAAEVSP--RIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEI---PVLNAD 225 +SS+ + + +G++T LT DHL+ H +E Y A K L + ++N D Sbjct: 189 VSSHALAQGRVLGCPFEVGVFTNLTQDHLDFHRDMEDYFAAKALLFSPDYLQGRAIINTD 248 Query: 226 DADIRQHCGHWSRSCIRWV---SCGDHSPEGGNPQLTVNAQGWVSRGEQHLF--PADSL- 279 D + + I+ + +S + G ++ G+ G PA + Sbjct: 249 DP--------YGQRLIKQLPPERVWSYSVQAGTADFCLSDLGYEPTGVSGKLHTPAGEVE 300 Query: 280 ---PLPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE 322 PL G++N N+L AAAL GV I L F GVP R+E Sbjct: 301 FRSPLVGQYNLANLLAAVAAALHLGVDLQLIANVLPQFAGVPGRME 346 >Chro_1549 UDP-N-acetylmuramate--L-alanine ligase Length = 498 Score = 39.3 bits (90), Expect = 6e-04, Method: Compositional matrix adjust. Identities = 51/227 (22%), Positives = 92/227 (40%), Gaps = 16/227 (7%) Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMS 172 I + GT+GKTT + + ++L AGL+ + VG E+ ++V E Sbjct: 152 IAVAGTHGKTTTSSAIGYMLLKAGLDPTII--VG---GEVKAWEGNARLGNSQYLVAEAD 206 Query: 173 SYQIEAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQH 232 + +++ +IG+ T + DH + + ++E + ++ + V D +R Sbjct: 207 ESD-GSLVKLACQIGVVTNIELDHPDHYKSLEQVVKTFQTFAQQCQHVVGCIDCQTVRDR 265 Query: 233 CGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVS----RGEQHLFPADSLPLPGEHNRQ 288 + I + + + + + A G ++ RG++ LPL G+HN Sbjct: 266 L----QPTISYSLNPEMAADYTVSNVIYGADGTIATVWERGKE--LGQLRLPLLGQHNLS 319 Query: 289 NMLLVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFND 335 N L A GV + I +L F G R E G V I +D Sbjct: 320 NALAAIAVGRLLGVEFAAIADSLTTFEGARRRFELRGVVNDIQFIDD 366 >Chro_5104 protein of unknown function DUF1727 Length = 452 Score = 38.9 bits (89), Expect = 8e-04, Method: Compositional matrix adjust. Identities = 78/327 (23%), Positives = 123/327 (37%), Gaps = 87/327 (26%) Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALE------VLQGTRP---- 162 I I GTNGKTT + LL+ +L G V ++AA LE +LQ T P Sbjct: 59 ILIAGTNGKTTTSLLLRTMLERQGWR------VVHNAAGANLENGLMTALLQNTNPIGTL 112 Query: 163 KPDWVVMEMSSYQI-EAAAEVSPRIGIWTTLTPDHLERHGTIEAY-----RAIKRGLLER 216 D+ ++E+ + + A + PR+ + L D L+R+G ++ +AI L Sbjct: 113 DADFAILEVDENIVPKVLAPLQPRLILALNLFRDQLDRYGEVDTISRRWGQAI--APLPS 170 Query: 217 SEIPVLNADDADIRQHCGHWSRSCIRW------------------VSC------------ 246 I V NADD + S++ + + + C Sbjct: 171 ETIIVANADDPTLAYMGQQLSQNVLFFGLSEPEHYLDEIPHAVDSIYCPSCGTRLVYQGV 230 Query: 247 -----GDHS-PEGG--NPQLTVNAQGWVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAAL 298 GD++ P G P L + ++ W EQ L G +N+ N L +A Sbjct: 231 YLSHQGDYNCPNCGFHKPSLAIASREW----EQILI--------GLYNKYNTLAAVLSAQ 278 Query: 299 AAGVSASTIEAALRCFTGVPHRLEPLGSVGG---IPVFNDSKATNYDAAAV----GLRSV 351 GV + I + F R E L G I + + N AV G R + Sbjct: 279 QLGVDEAIIRDTIANFQAAFGRAEELQVRGKHVRILLSKNPVGMNETIRAVNEVRGERQI 338 Query: 352 Q------APVVLLAGGQTKKGDAVPWL 372 + APV+++ + G V W+ Sbjct: 339 EAESRQLAPVLMVLNDRIPDGTDVSWI 365 >Chro_5574 amine oxidase Length = 500 Score = 32.3 bits (72), Expect = 0.10, Method: Compositional matrix adjust. Identities = 16/34 (47%), Positives = 21/34 (61%) Query: 3 RSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEP 36 + VV+G G +G GA L QGY+V +LE G P Sbjct: 7 KVVVIGAGWAGLGATYRLAKQGYNVTLLEAGAYP 40 >Chro_2386 ABC-2 type transporter Length = 259 Score = 31.2 bits (69), Expect = 0.16, Method: Compositional matrix adjust. Identities = 16/41 (39%), Positives = 22/41 (53%) Query: 274 FPADSLPLPGEHNRQNMLLVTAAALAAGVSASTIEAALRCF 314 +P S+ L + + L+TA AL GV ST+ A L CF Sbjct: 132 YPLGSILLAVVYLFLQLSLITAIALVLGVFTSTVLATLLCF 172 >Chro_5111 C-3',4' desaturase CrtD Length = 504 Score = 31.6 bits (70), Expect = 0.19, Method: Compositional matrix adjust. Identities = 20/55 (36%), Positives = 29/55 (52%), Gaps = 2/55 (3%) Query: 2 SRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGC 56 SR VV+G G G AA LL +GY V+VL++ P A +++ T +G Sbjct: 11 SRVVVIGAGIGGLTAAALLARRGYQVLVLDQAIVPG--GCASTFKRKGFTFDVGA 63 >Chro_0203 short-chain dehydrogenase/reductase SDR Length = 248 Score = 29.6 bits (65), Expect = 0.57, Method: Compositional matrix adjust. Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 2/40 (5%) Query: 11 RSGSGAARLLQAQGYDV--VVLERGDEPALRDKAHALRQQ 48 + G AA L+A+G+DV V ++ DE +++D A + +Q Sbjct: 39 QRGEAAAETLRAEGFDVHFVPIDINDESSIKDAAETVARQ 78 >Chro_2389 amine oxidase Length = 454 Score = 29.6 bits (65), Expect = 0.60, Method: Compositional matrix adjust. Identities = 20/75 (26%), Positives = 37/75 (49%), Gaps = 9/75 (12%) Query: 3 RSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQ-QNITIQLGCPLELD 61 + +++G G SG A LL +G D+ VLE R + + + +N+ ++LG Sbjct: 35 KVIIIGAGLSGLAAGYLLSQKGIDLTVLEARSRLGGRVYSQTIDENENLAVELGA----- 89 Query: 62 SFTPWLDSAGEVVIS 76 W+ ++ + VIS Sbjct: 90 ---EWIGASHQRVIS 101 Database: GCA_000317125.1P Posted date: Aug 27, 2016 10:57 PM Number of letters in database: 1,839,647 Number of sequences in database: 5752 Lambda K H 0.319 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 5752 Number of Hits to DB: 1,727,488 Number of extensions: 74482 Number of successful extensions: 274 Number of sequences better than 1.0: 16 Number of HSP's gapped: 265 Number of HSP's successfully gapped: 16 Length of query: 462 Length of database: 1,839,647 Length adjustment: 89 Effective length of query: 373 Effective length of database: 1,327,719 Effective search space: 495239187 Effective search space used: 495239187 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 59 (27.3 bits)