BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
synthetase
(462 letters)
Database: GCA_000312725.1P
3994 sequences; 1,117,529 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MICAE_1250005 murD UDP-N-acetylmuramoylalanine--D-glutamate ligase 345 e-114
MICAE_400016 murE UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, ... 50 2e-07
MICAE_290002 murC UDP-N-acetylmuramate--L-alanine ligase 49 3e-07
MICAE_90003 UDP-N-acetylmuramyl tripeptide synthetase like 39 7e-04
MICAE_800005 cphA Cyanophycin synthetase 38 0.001
MICAE_1520028 murF UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- ... 37 0.003
MICAE_650004 Genome sequencing data, contig C319 32 0.091
MICAE_980006 NAD-dependent epimerase/dehydratase 31 0.10
MICAE_710013 iam Isoamylase 30 0.36
MICAE_1740021 putative amidase 28 0.79
>MICAE_1250005 murD UDP-N-acetylmuramoylalanine--D-glutamate ligase
Length = 449
Score = 345 bits (885), Expect = e-114, Method: Compositional matrix adjust.
Identities = 201/458 (43%), Positives = 278/458 (60%), Gaps = 16/458 (3%)
Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLEL 60
M+++ ++GLGRSG AAR L+ G+ V + +R D P L+ L ++ I + LG L L
Sbjct: 1 MAKAAIIGLGRSGIAAARCLKRDGWQVTLSDRSDSPGLQATKTNLEREGIIVNLGQNLSL 60
Query: 61 DSFTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNG 120
++ D +V+SPG+ WD L R G GE+ +AW L+ PW+GITGTNG
Sbjct: 61 EAS----DLPNLIVVSPGVPWDAPILITAREKGIDTIGELELAWRYLQSSPWLGITGTNG 116
Query: 121 KTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAA 180
KTT T L + AGL AP GN+GY+A ELAL+ Q DW++ E+SSYQIE++
Sbjct: 117 KTTTTALCAAIFQKAGLNAPSCGNIGYAACELALKADQ-----YDWIIAEISSYQIESSR 171
Query: 181 EVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQ-HCGHWSRS 239
++SP+IGIWTT TPDHL RH T+E Y IK LL+RS+ +LN DD +RQ W ++
Sbjct: 172 DLSPKIGIWTTFTPDHLSRHQTLENYYQIKASLLQRSDRQILNGDDPYLRQIGVSQWQQA 231
Query: 240 CIRWVSCGDHSPEGGNPQLTVNAQ-GWVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAAL 298
W S G+P V Q W+ + + P + L + G HN+QN+L+ AA+
Sbjct: 232 --YWTSVQGKDALLGDPSRGVYLQDNWIVAFGELIAPVNLLKMVGSHNQQNLLMAVAASR 289
Query: 299 AAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKATNYDAAAVGLRSVQAPVVLL 358
AG+ I A+ F GV HRLE + + G+ NDSKATNYDAAAVGL+SV P +L+
Sbjct: 290 LAGIEKKAITEAIATFPGVAHRLEYICTHKGLDFINDSKATNYDAAAVGLQSVPGPAILI 349
Query: 359 AGGQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLIETSGFAGPMIHCAD-LDSAVSQ 417
AGG+ K GD W+ I+++VA V+L G+ A + A ++++G+ C + +D AV +
Sbjct: 350 AGGEAKAGDDRAWIAEIKAKVATVLLIGDAAADFARRLQSAGYQD--YECVETMDRAVQR 407
Query: 418 ALSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQL 455
A L +A VLLSPACASFDQY +FE RGDHFRQL
Sbjct: 408 AAELGLAKEAKVVLLSPACASFDQYQSFEHRGDHFRQL 445
>MICAE_400016 murE UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
6-diaminopimelate ligase
Length = 496
Score = 49.7 bits (117), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 62/222 (27%), Positives = 97/222 (43%), Gaps = 30/222 (13%)
Query: 126 HLLQHVLAHAGLEAPMAGNV-----GYS---------AAELALEVLQGTRPKPDWVVMEM 171
HL+++ L+ AG+ + G + G+ A EL + + + VME+
Sbjct: 126 HLIEYFLSQAGIPTALFGTLYTRWQGFQETANHTTPFANELQASLAKAVSAGNEIAVMEV 185
Query: 172 SSYQIEAAAEV---SPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEI---PVLNAD 225
SS+ + A V + + ++T LT DHL+ H +E Y A K L + ++N D
Sbjct: 186 SSHAL-AQGRVQGCTFPVTVFTNLTRDHLDYHLDMEDYFAAKAKLFSADYLQGKAIINLD 244
Query: 226 DADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQG-----WVSRGEQHLFPADSLP 280
DA ++ S + S D S + L G RGE A + P
Sbjct: 245 DAYGQRLAASLSPQQVLTYSVSDASADFYTKDLQYQPTGVEGVMVTPRGEI----AFASP 300
Query: 281 LPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE 322
L G++N N+L AA L GV T+ A + F+GVP R+E
Sbjct: 301 LVGQYNLANLLAAVAAVLECGVDLETVIAQIPQFSGVPGRME 342
>MICAE_290002 murC UDP-N-acetylmuramate--L-alanine ligase
Length = 475
Score = 49.3 bits (116), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 66/275 (24%), Positives = 105/275 (38%), Gaps = 30/275 (10%)
Query: 72 EVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNGKTTVTHLLQHV 131
+V+ S I + A R G+ + V + I + GT+GKTT + L+ +V
Sbjct: 82 QVICSTAINDHNKEYQAARDLGYPMFHRSDVLAALIADYDSIAVAGTHGKTTTSSLIGYV 141
Query: 132 LAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAAEVSPRIGIWTT 191
L AGL+ + A E + QG ++V E + + +P+IG+ T
Sbjct: 142 LLEAGLDPTIIVGGEVDAWEGNARLGQGR-----FLVAEADESD-GSLTKHAPKIGVITN 195
Query: 192 LTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQHCGHWSRSCIRWVSCGDHSP 251
+ DH + + + + +I V D I +H R I + P
Sbjct: 196 IELDHPDHYQNLSEVIDTFHEFAHQCQILVACLDCDTIAEHF----RPTISY----SLDP 247
Query: 252 EGGNPQLTVNAQGWVSRGEQHLFPAD-----------SLPLPGEHNRQNMLLVTAAALAA 300
E G TV+ + EQ + A + +PG+HN N L V A
Sbjct: 248 EKG-ADYTVSEIIY----EQGMMKASVWEKGSYLGQMEVKIPGQHNISNALAVVAIGRYL 302
Query: 301 GVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFND 335
G+ + I A+ F G R E G GI +D
Sbjct: 303 GLEFAVIADAIATFAGAKRRFEHKGEANGITFIDD 337
>MICAE_90003 UDP-N-acetylmuramyl tripeptide synthetase like
Length = 445
Score = 38.5 bits (88), Expect = 7e-04, Method: Compositional matrix adjust.
Identities = 45/151 (29%), Positives = 65/151 (43%), Gaps = 17/151 (11%)
Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNV-------GYSAAELALEVLQGTRPKPD 165
I + GTNGKTT + LL+ +L G A + N G A LA L GT K D
Sbjct: 59 ILVVGTNGKTTTSLLLRTILERQG--AKVTHNTTGANLINGLITALLADANLFGTL-KAD 115
Query: 166 WVVMEMSSYQIE-AAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGL---LERSEIPV 221
+ ++E+ + + PR + L D L+R+G ++ +G L + +
Sbjct: 116 YAILEVDENILPLVLKDCRPRAILALNLFRDQLDRYGEVDTISRRWQGAISPLPTDTLVI 175
Query: 222 LNADDADIRQHCGHWSRSCIRWVSCGDHSPE 252
LNADD + H G R V G PE
Sbjct: 176 LNADDPTL-SHLGQQLPQ--RVVYFGLSEPE 203
>MICAE_800005 cphA Cyanophycin synthetase
Length = 877
Score = 38.1 bits (87), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 54/237 (22%), Positives = 83/237 (35%), Gaps = 35/237 (14%)
Query: 110 IPWIGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVM 169
+P + ITGTNGKTT + L+ H+ G G + +E +G P M
Sbjct: 488 VPILAITGTNGKTTTSRLISHICRQTGKVVGFTTTDGVYIDDYLVE--KGDNTGPYSASM 545
Query: 170 EMSSYQIEAAAEVSPR--------------IGIWTTLTPDHL--ERHGTIEAYRAIKRGL 213
+ +E A + R +G+ + DHL TIE +K +
Sbjct: 546 ILKDPTVEIAVLETARGGILRSGLAFNQCDVGVVLNVAADHLGIGDIDTIEQMAKVKSVV 605
Query: 214 LERSE---IPVLNADD-------ADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQ 263
E VLNADD ++ ++S S + DH GG + N
Sbjct: 606 AEVVSAEGYAVLNADDPLTASMAEKVKGRVAYFSMSPDNPI-IHDHIRRGGMAAIYENGY 664
Query: 264 GWVSRGEQHLFPADSLPLPGEHNRQ------NMLLVTAAALAAGVSASTIEAALRCF 314
+ GE L +++ +P N L A G+ I +R F
Sbjct: 665 LSILEGEWTLRIEEAVNIPVTMQGMAPFMIANALAACLATFVQGIDIELIRQGVRTF 721
>MICAE_1520028 murF UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Length = 456
Score = 36.6 bits (83), Expect = 0.003, Method: Compositional matrix adjust.
Identities = 57/224 (25%), Positives = 86/224 (38%), Gaps = 32/224 (14%)
Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLEAPMAGN----VGYSAAELALEVLQG 159
W IP I ITG+ GKTT L+ VLA G N +G L L+
Sbjct: 104 WRNQFTIPVIAITGSVGKTTTKELIASVLATQGTVHKTLANYNNEIGVPKTLLELD---- 159
Query: 160 TRPKPDWVVMEMSSY---QIEAAAEVS-PRIGIWTTLTPDHLERHGT---IEAYRAIKRG 212
D V+EM +I A+++ P I + T + H+ R G+ I +
Sbjct: 160 --ETHDSAVIEMGMRGRGEIALLAQITQPTIAVITNVGTAHIGRLGSEAAIAEAKCELLS 217
Query: 213 LLERSEIPVLNADDADIRQHCGH-WSRSCIRW-VSCGDHSPEGGN-PQLTVNAQGWVSRG 269
S I VLN D + + W I + S GD E + L VN +
Sbjct: 218 ESPSSSIAVLNHDHPLLTETAKRVWQGETITYGFSGGDIVGELVDLTTLRVNGLDF---- 273
Query: 270 EQHLFPADSLPLPGEHNRQNMLLVTAAALAAGVSASTIEAALRC 313
+LPL G H+ N + A A G+ ++++ ++
Sbjct: 274 --------NLPLSGRHHALNFMAALAVAKILGIDWTSLQQGIKV 309
>MICAE_650004 Genome sequencing data, contig C319
Length = 448
Score = 31.6 bits (70), Expect = 0.091, Method: Compositional matrix adjust.
Identities = 15/44 (34%), Positives = 25/44 (56%)
Query: 95 RVRGEMSVAWDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLE 138
R++ + + + IP I +TGTNGK +V L +L+ AG +
Sbjct: 54 RIKNLLEILGNPQDKIPIIHVTGTNGKGSVCAYLSSILSTAGYK 97
>MICAE_980006 NAD-dependent epimerase/dehydratase
Length = 352
Score = 31.2 bits (69), Expect = 0.10, Method: Compositional matrix adjust.
Identities = 22/77 (28%), Positives = 35/77 (45%), Gaps = 7/77 (9%)
Query: 375 IQSQVAGVVLFGEGAGELADLIETSGFAGPMIHCAD----LDSAVS---QALSLARNCQA 427
IQ V F GE + +I + A ++H D LD+ V + L A+NC
Sbjct: 81 IQFHSGDVRSFEFPEGEFSYIIHAATDASSLVHQEDRLFVLDTTVQGTRRVLDFAKNCGT 140
Query: 428 NSVLLSPACASFDQYPN 444
+LL+ + A + Q P+
Sbjct: 141 KKLLLTSSGAIYGQQPS 157
>MICAE_710013 iam Isoamylase
Length = 789
Score = 30.0 bits (66), Expect = 0.36, Method: Compositional matrix adjust.
Identities = 20/50 (40%), Positives = 26/50 (52%), Gaps = 2/50 (4%)
Query: 24 GYDVV-VLERGDEPALRDKAHALRQQ-NITIQLGCPLELDSFTPWLDSAG 71
G+DVV + DE L D A Q N + + C L LDS T WL++ G
Sbjct: 358 GFDVVEYYQLTDEHYLVDGATGCGNQLNFSHSVTCNLVLDSLTYWLETMG 407
>MICAE_1740021 putative amidase
Length = 465
Score = 28.5 bits (62), Expect = 0.79, Method: Compositional matrix adjust.
Identities = 27/85 (31%), Positives = 37/85 (43%), Gaps = 6/85 (7%)
Query: 292 LVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKA-----TNYDAAAV 346
L TA+A +S T A L F GVP ++ L +V G+PV A YD V
Sbjct: 54 LATASAQTEQLSQITDTAELPPFFGVPTAIKDLNAVAGMPVSYGVAALQGNIAQYDEGIV 113
Query: 347 GLRSVQAPVVLLAGGQTKKGDAVPW 371
R A +LL T + + P+
Sbjct: 114 -TRMKMAGFILLGKTATSQLGSFPY 137
Database: GCA_000312725.1P
Posted date: Aug 27, 2016 10:38 PM
Number of letters in database: 1,117,529
Number of sequences in database: 3994
Lambda K H
0.319 0.134 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3994
Number of Hits to DB: 1,036,328
Number of extensions: 43673
Number of successful extensions: 154
Number of sequences better than 1.0: 12
Number of HSP's gapped: 149
Number of HSP's successfully gapped: 13
Length of query: 462
Length of database: 1,117,529
Length adjustment: 85
Effective length of query: 377
Effective length of database: 778,039
Effective search space: 293320703
Effective search space used: 293320703
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)