BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
synthetase
(462 letters)
Database: GCA_000297435.1P
4434 sequences; 1,223,634 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MICAI_2130032 murD UDP-N-acetylmuramoylalanine--D-glutamate ligase 345 e-114
MICAI_560041 murC UDP-N-acetylmuramate--L-alanine ligase 51 1e-07
MICAI_1470020 murE UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,... 47 1e-06
MICAI_2670017 murF UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- ... 39 5e-04
MICAI_3180004 UDP-N-acetylmuramyl tripeptide synthetase like 38 0.001
MICAI_650002 cphA Cyanophycin synthetase 38 0.001
MICAI_2990004 folC Folylpolyglutamate synthase 32 0.11
MICAI_2150007 putative amidase 30 0.22
MICAI_1730025 conserved hypothetical protein 29 0.67
>MICAI_2130032 murD UDP-N-acetylmuramoylalanine--D-glutamate ligase
Length = 449
Score = 345 bits (885), Expect = e-114, Method: Compositional matrix adjust.
Identities = 203/458 (44%), Positives = 281/458 (61%), Gaps = 16/458 (3%)
Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLEL 60
M+++ ++GLGRSG AAR L+ G+ V + +R D P L++ L ++ I + LG L L
Sbjct: 1 MAKAAIIGLGRSGIAAARCLKRDGWQVTLSDRSDAPGLQETKTNLEREGIMVNLGQNLSL 60
Query: 61 DSFTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNG 120
++ D +V+SPG+ WD L R G GE+ +AW L+ PW+GITGTNG
Sbjct: 61 EAS----DLPNLIVVSPGVPWDAPILITAREKGIDTIGELELAWRYLQSSPWLGITGTNG 116
Query: 121 KTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAA 180
KTT T L + AGL AP GN+GY+A ELAL+ K DW++ E+SSYQIE++
Sbjct: 117 KTTTTALCAAIFQKAGLNAPSCGNIGYAACELALKA-----DKYDWIIAEISSYQIESSR 171
Query: 181 EVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQ-HCGHWSRS 239
++SP+IGIWTT TPDHL RH T+E Y IK LL+RS+ +LN DD +RQ W ++
Sbjct: 172 DLSPKIGIWTTFTPDHLNRHQTLENYYRIKASLLQRSDRQILNGDDPYLRQIGVSQWQQA 231
Query: 240 CIRWVSCGDHSPEGGNPQLTVNAQ-GWVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAAL 298
W S G+P V Q W+ + + P + L + G HN+QN+L+ AAA
Sbjct: 232 --YWTSVQGKEALLGDPSRGVYLQDNWIVAFGELIAPVNLLKMVGSHNQQNLLMAVAAAR 289
Query: 299 AAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKATNYDAAAVGLRSVQAPVVLL 358
AG+ I A+ F GV HRLE + + G+ NDSKATNYDAAAVGL+SV +P +L+
Sbjct: 290 LAGIEKKAITEAIATFPGVAHRLEYICTYKGLDFINDSKATNYDAAAVGLKSVPSPAILI 349
Query: 359 AGGQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLIETSGFAGPMIHCAD-LDSAVSQ 417
AGG+ K GD W+ I+++VA V+L G+ A + A ++++G+ C + +D AV +
Sbjct: 350 AGGEAKAGDDRAWIAEIKAKVATVLLIGDAAADFARRLQSAGYQD--YECVETMDRAVQR 407
Query: 418 ALSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQL 455
A LA +A VLLSPACASFDQY +FE RGDHFRQL
Sbjct: 408 AAELAAAKEAKVVLLSPACASFDQYQSFEHRGDHFRQL 445
>MICAI_560041 murC UDP-N-acetylmuramate--L-alanine ligase
Length = 475
Score = 50.8 bits (120), Expect = 1e-07, Method: Compositional matrix adjust.
Identities = 65/273 (23%), Positives = 102/273 (37%), Gaps = 26/273 (9%)
Query: 72 EVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTNGKTTVTHLLQHV 131
+V+ S I + A R G+ + V + I + GT+GKTT + L+ +V
Sbjct: 82 QVICSTAINDHNKEYQAARDLGYPIFHRSDVLAALIADYDSIAVAGTHGKTTTSSLIGYV 141
Query: 132 LAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIEAAAEVSPRIGIWTT 191
L AGL+ + A E + QG ++V E + + +P+IG+ T
Sbjct: 142 LLEAGLDPTIIVGGEVDAWEGNARLGQGR-----FLVAEADESD-GSLTKHAPKIGVITN 195
Query: 192 LTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQHCGHWSRSCIRWVSCGDHSP 251
+ DH + + + + +I V D I +H R I + P
Sbjct: 196 IELDHPDHYHNLSEVIDTFHEFAHQCQILVACLDCDTIAEHF----RPTISY----SLDP 247
Query: 252 EGG---------NPQLTVNAQGWVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAALAAGV 302
E G Q + A W G + +PG+HN N L V A G+
Sbjct: 248 EKGADYTVSEIIYEQGMMKASVWEKGG---YLGQMEVKIPGQHNISNALAVVAIGRYLGL 304
Query: 303 SASTIEAALRCFTGVPHRLEPLGSVGGIPVFND 335
+ I A+ F G R E G GI +D
Sbjct: 305 EFAVIADAIATFAGAKRRFEHKGEANGITFIDD 337
>MICAI_1470020 murE UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
6-diaminopimelate ligase
Length = 496
Score = 47.4 bits (111), Expect = 1e-06, Method: Compositional matrix adjust.
Identities = 61/222 (27%), Positives = 97/222 (43%), Gaps = 30/222 (13%)
Query: 126 HLLQHVLAHAGLEAPMAGNV-----GYS---------AAELALEVLQGTRPKPDWVVMEM 171
HL+++ L+ AG+ + G + G+ A EL + + + VME+
Sbjct: 126 HLIEYFLSQAGIATALFGTLYTRWQGFQETANHTTPFANELQASLAKAVSAGNEIAVMEV 185
Query: 172 SSYQIEAAAEV---SPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEI---PVLNAD 225
SS+ + A V + + ++T LT DHL+ H +E Y A K L + ++N D
Sbjct: 186 SSHAL-AQGRVQGCTFPVTVFTNLTRDHLDYHLDMEDYFAAKAKLFSPDYLQGKAIINLD 244
Query: 226 DADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQG-----WVSRGEQHLFPADSLP 280
DA ++ S + S D S + L G +GE A + P
Sbjct: 245 DAYGQRLAASLSPQQVLTYSVSDASADFYTKDLHYQPTGVEGVMVTPQGEI----AFASP 300
Query: 281 LPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE 322
L G++N N+L AA L GV T+ A + F+GVP R+E
Sbjct: 301 LVGQYNLANLLAAVAAVLECGVDLETVIAQIPQFSGVPGRME 342
>MICAI_2670017 murF UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Length = 456
Score = 38.9 bits (89), Expect = 5e-04, Method: Compositional matrix adjust.
Identities = 59/223 (26%), Positives = 88/223 (39%), Gaps = 32/223 (14%)
Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLEAPMAGN----VGYSAAELALEVLQG 159
W IP I ITG+ GKTT L+ +LA G N +G L L+
Sbjct: 104 WRNQFTIPVIAITGSVGKTTTKELIASLLASRGKVHKTLANYNNEIGVPKTLLELD---- 159
Query: 160 TRPKPDWVVMEMSSY---QIEAAAEVS-PRIGIWTTLTPDHLERHGTIEAYRAIKRGLLE 215
D V+EM +I A+++ P I + T + H+ R G+ A K LL
Sbjct: 160 --ETHDSAVIEMGMRGPGEIALLAQIAQPTIAVITNVGTAHIGRLGSEAAIAEAKCELLA 217
Query: 216 R---SEIPVLNADDADIRQHCGH-WSRSCIRW-VSCGDHSPEGGN-PQLTVNAQGWVSRG 269
S I VLN D + + W I + S GD E + L VN +
Sbjct: 218 ESPSSSIAVLNHDHPLLTETAKRVWQGETITYGFSGGDIVGELLDLTTLRVNGLDF---- 273
Query: 270 EQHLFPADSLPLPGEHNRQNMLLVTAAALAAGVSASTIEAALR 312
+LPL G H+ N + A A G+ ++++ ++
Sbjct: 274 --------NLPLSGRHHALNFMAALAVAKILGIDWTSLQEGIK 308
>MICAI_3180004 UDP-N-acetylmuramyl tripeptide synthetase like
Length = 445
Score = 38.1 bits (87), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 45/151 (29%), Positives = 65/151 (43%), Gaps = 17/151 (11%)
Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNV-------GYSAAELALEVLQGTRPKPD 165
I + GTNGKTT + LL+ +L G A + N G A LA L GT K D
Sbjct: 59 ILVVGTNGKTTTSLLLRTILERQG--AKVTHNTTGANLINGLITALLADANLFGTL-KAD 115
Query: 166 WVVMEMSSYQIE-AAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGL---LERSEIPV 221
+ ++E+ + + PR + L D L+R+G ++ +G L + +
Sbjct: 116 YAILEVDENILPLVLKDCRPRAILALNLFRDQLDRYGEVDTISRRWQGAISPLPTDTLVI 175
Query: 222 LNADDADIRQHCGHWSRSCIRWVSCGDHSPE 252
LNADD + H G R V G PE
Sbjct: 176 LNADDPTL-SHLGQQLPQ--RVVYFGLSEPE 203
>MICAI_650002 cphA Cyanophycin synthetase
Length = 877
Score = 38.1 bits (87), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 54/237 (22%), Positives = 83/237 (35%), Gaps = 35/237 (14%)
Query: 110 IPWIGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVM 169
+P + ITGTNGKTT + L+ H+ G G + +E +G P M
Sbjct: 488 VPILAITGTNGKTTTSRLISHICRQTGKVVGFTTTDGVYIDDYLVE--KGDNTGPYSASM 545
Query: 170 EMSSYQIEAAAEVSPR--------------IGIWTTLTPDHL--ERHGTIEAYRAIKRGL 213
+ +E A + R +G+ + DHL TIE +K +
Sbjct: 546 ILKDPTVEIAVLETARGGILRSGLAFNQCDVGVVLNVAADHLGIGDIDTIEQMAKVKSVV 605
Query: 214 LERSE---IPVLNADD-------ADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQ 263
E VLNADD ++ ++S S + DH GG + N
Sbjct: 606 AEVVSAEGYAVLNADDPLTASMAEKVKGRVAYFSMSPDNPI-IHDHIRRGGMAAIYENGY 664
Query: 264 GWVSRGEQHLFPADSLPLPGEHNRQ------NMLLVTAAALAAGVSASTIEAALRCF 314
+ GE L +++ +P N L A G+ I +R F
Sbjct: 665 LSILEGEWTLRIEEAVNIPVTMQGMAPFMIANALAACLATFVQGIDIELIRQGVRTF 721
>MICAI_2990004 folC Folylpolyglutamate synthase
Length = 415
Score = 31.6 bits (70), Expect = 0.11, Method: Compositional matrix adjust.
Identities = 15/44 (34%), Positives = 25/44 (56%)
Query: 95 RVRGEMSVAWDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLE 138
R++ + + + IP I +TGTNGK +V L +L+ AG +
Sbjct: 21 RIKNLLEILGNPQDKIPIIHVTGTNGKGSVCAYLSSILSTAGYK 64
>MICAI_2150007 putative amidase
Length = 465
Score = 30.4 bits (67), Expect = 0.22, Method: Compositional matrix adjust.
Identities = 27/85 (31%), Positives = 37/85 (43%), Gaps = 6/85 (7%)
Query: 292 LVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKA-----TNYDAAAV 346
L TA+A +S T A L F GVP ++ L +V G+PV A YD V
Sbjct: 54 LATASAQTEQLSQITATAELPTFFGVPTAIKDLNAVAGMPVSYGVAALQGNIAQYDEGIV 113
Query: 347 GLRSVQAPVVLLAGGQTKKGDAVPW 371
R A +LL T + + P+
Sbjct: 114 A-RMKMAGFILLGKTATSQLGSFPY 137
>MICAI_1730025 conserved hypothetical protein
Length = 460
Score = 28.9 bits (63), Expect = 0.67, Method: Compositional matrix adjust.
Identities = 15/27 (55%), Positives = 20/27 (74%)
Query: 5 VVVGLGRSGSGAARLLQAQGYDVVVLE 31
+VVG G +G AAR LQ QG+ V+V+E
Sbjct: 12 IVVGAGLAGLIAARNLQRQGHQVLVIE 38
Database: GCA_000297435.1P
Posted date: Aug 27, 2016 10:58 PM
Number of letters in database: 1,223,634
Number of sequences in database: 4434
Lambda K H
0.319 0.134 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4434
Number of Hits to DB: 1,131,776
Number of extensions: 47738
Number of successful extensions: 163
Number of sequences better than 1.0: 11
Number of HSP's gapped: 158
Number of HSP's successfully gapped: 12
Length of query: 462
Length of database: 1,223,634
Length adjustment: 86
Effective length of query: 376
Effective length of database: 842,310
Effective search space: 316708560
Effective search space used: 316708560
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)