cyanobase / GCA_000153285.1 - WH7805

BLASTP 2.2.26 [Sep-21-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase (462 letters) Database: GCA_000155595.1P 5586 sequences; 1,647,188 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value S7335_4460 UDP-N-acetylmuramoylalanine--D-glutamate ligase 319 e-104 S7335_4976 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ... 54 2e-08 S7335_4962 UDP-N-acetylmuramyl-tripeptide synthetases subfamily 52 8e-08 S7335_3656 UDP-N-acetylmuramate--alanine ligase 44 2e-05 S7335_4309 TrkA-N domain family 36 0.007 S7335_2806 FAD dependent oxidoreductase, putative 32 0.13 S7335_3267 FAD dependent oxidoreductase, putative 31 0.29 S7335_2705 Pyridine nucleotide-disulphide oxidoreductase, putative 30 0.35 S7335_4565 TrkA-N domain superfamily 29 0.52 S7335_385 FAD dependent oxidoreductase, putative 29 0.71 8826b2b642128dfe3e628eb550c96408

>S7335_4460 UDP-N-acetylmuramoylalanine--D-glutamate ligase Length = 457 Score = 319 bits (817), Expect = e-104, Method: Compositional matrix adjust. Identities = 204/460 (44%), Positives = 264/460 (57%), Gaps = 16/460 (3%) Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLEL 60 M + V+GLGRSG AAR+L QGY V + +RG + A L+ + IT++L Sbjct: 1 MKTAHVIGLGRSGIAAARVLARQGYVVTLSDRGAAQRHQSDARRLKDEGITVKLNY---- 56 Query: 61 DSFTPWL--DSAGEV-VISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITG 117 +F P + DS ++ V+SPG+ WD L R G GEM +AW ALR PW+GITG Sbjct: 57 -AFDPQVAKDSGLDLLVVSPGVPWDAPALVTARKLGIETLGEMELAWRALRDRPWVGITG 115 Query: 118 TNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMSSYQIE 177 TNGKTT T L+ + AGL AP GN+GY+A ELAL PDWV+ E+SSYQIE Sbjct: 116 TNGKTTTTALVAAIFKAAGLNAPACGNIGYAACELALT------ESPDWVIAELSSYQIE 169 Query: 178 AAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQHCGHWS 237 A+A ++P IGIWTT TPDHL RH T+E Y +IK LL RS+ ++N DD +++ S Sbjct: 170 ASASLAPTIGIWTTFTPDHLSRHKTVENYYSIKASLLARSQSVIINGDDLTLQRKAQEHS 229 Query: 238 RSCIRWVSCGDHSPEGGNPQLTVNAQGWVSRGEQHLFPADSLPLPGEHNRQNMLLVTAAA 297 S W T GWV + + +L + G HN+QN+LL AAA Sbjct: 230 SSRHHWTCIEGKDALAPIVVDTYIEDGWVIHKGEQIVETKALRMVGTHNQQNLLLAVAAA 289 Query: 298 LAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKATNYDAAAVGLRSVQAPVVL 357 AG+ I + F GV HRLE + + GI NDSKATNYDAA VGLRSV P++L Sbjct: 290 RLAGIDRRAIALGVASFPGVSHRLEHICTWRGIDFINDSKATNYDAAEVGLRSVAEPIIL 349 Query: 358 LAGGQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLIETSGFAG-PMIHCADLDSAVS 416 +AGG K GD W+ I+ + V+L G+ AG A +E + + ++ D A S Sbjct: 350 IAGGAQKDGDDTIWIDRIKEKAVAVLLVGDAAGPFAKRLEEADYHNYEIVQTIDRAVARS 409 Query: 417 QALSLARNCQ-ANSVLLSPACASFDQYPNFEARGDHFRQL 455 LS N Q +VLLSPACASFDQY NFE RGD FRQ+ Sbjct: 410 TELSTELNKQPIKTVLLSPACASFDQYLNFEVRGDDFRQI 449 >S7335_4976 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase subfamily Length = 455 Score = 53.5 bits (127), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 57/198 (28%), Positives = 85/198 (42%), Gaps = 25/198 (12%) Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPK 163 W + IP + ITG+ GKTT L+ L+ G N Y+ + L G + Sbjct: 104 WRSQCAIPVVAITGSVGKTTTKELVAAALSQFGTVFKTRAN--YNNEIGVPKTLLGLTTE 161 Query: 164 PDWVVMEMSSYQIEAAAEVS----PRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEI 219 D+ V+EM +E A ++ P + + T + H+ R G+ A K LL E+ Sbjct: 162 HDYAVVEMGMRGLEEIALLTQIAKPTVALITNVGTAHIGRLGSRAAIAQAKCELLR--EL 219 Query: 220 P-----VLNADDADIRQHCGH-WSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVSRGEQHL 273 P VLN DDA + WS + I + EGG+ + + + G + Sbjct: 220 PTDGTAVLNHDDARLMSTAARFWSGTQITY------GLEGGDVRGQIAGSDIIVDGVK-- 271 Query: 274 FPADSLPLPGEHNRQNML 291 P LPLPG HN N+L Sbjct: 272 IP---LPLPGRHNALNLL 286 >S7335_4962 UDP-N-acetylmuramyl-tripeptide synthetases subfamily Length = 534 Score = 51.6 bits (122), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 60/237 (25%), Positives = 91/237 (38%), Gaps = 60/237 (25%) Query: 126 HLLQHVLAHAGLEAPMAGNV-----GYS---------AAELALEVLQGTRPKPDWVVMEM 171 HL+++ L HA + + G + GY + +L E+ + + VVME+ Sbjct: 141 HLIEYFLNHAQQKTAILGTLYTRWPGYEKIAVHTTPFSIDLQAELASAHQAECQTVVMEV 200 Query: 172 SSYQIEAAA--EVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIP---VLNADD 226 SS+ + + + ++T LT DHL+ H ++ Y K L + ++N D Sbjct: 201 SSHALAQHRTWSIPFEVAVFTNLTQDHLDYHKDLDDYFEAKALLFSPDYLKGHAIVNQKD 260 Query: 227 A-----------------DIRQHCGHWSRSCIRW----VSCGDHSPEGGNPQLTVNAQGW 265 DI S +R+ VS H+P GN Sbjct: 261 PYGQKLIERIGKHKTLTYDITDRSADLYTSDLRYSPIGVSGTIHTP-AGNASF------- 312 Query: 266 VSRGEQHLFPADSLPLPGEHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE 322 S PL G+ N +N+L AAL GVS I AL F GVP R+E Sbjct: 313 ------------SSPLVGQFNLENLLAAVGAALHLGVSLDQIVVALPEFKGVPGRME 357 >S7335_3656 UDP-N-acetylmuramate--alanine ligase Length = 505 Score = 44.3 bits (103), Expect = 2e-05, Method: Compositional matrix adjust. Identities = 62/251 (24%), Positives = 103/251 (41%), Gaps = 21/251 (8%) Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMS 172 I I GT+GKTT + + ++L AG++ + +G + G + D+VV E Sbjct: 146 IAIAGTHGKTTTSSITGYLLLKAGVDPTII--IGGEVSAWCGNAYVG---QSDYVVAEAD 200 Query: 173 SYQIEAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQH 232 + ++S +IG+ T + DH + + +++ AI + + + V + D +R Sbjct: 201 ESD-GSLVKLSSQIGVITNIELDHPDHYTSLDQVVAIFDQFVTKCKTVVGSIDCPTVRDR 259 Query: 233 CGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVS----RGEQHLFPADSLPLPGEHNRQ 288 + I + + + + A G + RG+ L ++PL G HN Sbjct: 260 I----QPTISYSVEAEAKADYKAINIVETADGMTADILERGK--LLGNLTIPLLGLHNLS 313 Query: 289 NMLLVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFND-----SKATNYDA 343 N L A A VS +I+ L F G R E G I +D S+ A Sbjct: 314 NTLAAIAVARLLHVSFESIQTHLPNFKGARRRFEHRGFHNEILFVDDYAHHPSEIKATLA 373 Query: 344 AAVGLRSVQAP 354 AA RS Q P Sbjct: 374 AAQVQRSQQLP 384 >S7335_4309 TrkA-N domain family Length = 563 Score = 35.8 bits (81), Expect = 0.007, Method: Compositional matrix adjust. Identities = 24/61 (39%), Positives = 31/61 (50%), Gaps = 6/61 (9%) Query: 5 VVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLG---CPLELD 61 ++ GLG G A+ L AQGY VVV+ER D+ A R Q I + L PL L Sbjct: 339 IICGLGGVGIKTAQQLLAQGYKVVVIERDDDSRFLSMA---RSQQIPVILADASLPLTLQ 395 Query: 62 S 62 + Sbjct: 396 T 396 >S7335_2806 FAD dependent oxidoreductase, putative Length = 382 Score = 31.6 bits (70), Expect = 0.13, Method: Compositional matrix adjust. Identities = 39/174 (22%), Positives = 71/174 (40%), Gaps = 28/174 (16%) Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRD-------------------- 40 M ++VG G +GS AA L G V++LE+ + P + Sbjct: 1 MFDCIIVGAGPAGSTAAYHLAKAGCSVLLLEKAELPRYKPCTGAVSPSVADWFDFDFSPA 60 Query: 41 KAHALRQQNITIQLGCPLELDSFTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEM 100 A +RQ T +LG ++ + T + S V +D + + G +V+ + Sbjct: 61 IASEVRQVRYTWKLGDAIDAELETDPIWSVDRKV------FDQFLVEQAQLAGAKVQDQT 114 Query: 101 SVAWDALRGIPW--IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAEL 152 +V A G W ++G + ++V+A G + P+AG +G+ +L Sbjct: 115 TVTGVAFEGDHWEVSTVSGVERRLAERLTGRYVIAADGAKGPLAGWLGFREHKL 168 >S7335_3267 FAD dependent oxidoreductase, putative Length = 513 Score = 30.8 bits (68), Expect = 0.29, Method: Compositional matrix adjust. Identities = 17/35 (48%), Positives = 20/35 (57%) Query: 2 SRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEP 36 + VVVG G +G GA L QGY V +LE G P Sbjct: 18 KKVVVVGAGWAGLGATYHLAKQGYAVTLLEAGAFP 52 >S7335_2705 Pyridine nucleotide-disulphide oxidoreductase, putative Length = 540 Score = 30.4 bits (67), Expect = 0.35, Method: Compositional matrix adjust. Identities = 15/31 (48%), Positives = 20/31 (64%) Query: 3 RSVVVGLGRSGSGAARLLQAQGYDVVVLERG 33 R +V+G+G G AA LL G+ +VLERG Sbjct: 98 RPIVIGVGPCGMFAALLLAQMGFRPIVLERG 128 >S7335_4565 TrkA-N domain superfamily Length = 156 Score = 28.9 bits (63), Expect = 0.52, Method: Compositional matrix adjust. Identities = 15/28 (53%), Positives = 20/28 (71%) Query: 5 VVVGLGRSGSGAARLLQAQGYDVVVLER 32 V+VG GR GS A L +QG+ VVV+E+ Sbjct: 17 VIVGCGRLGSLLAGQLSSQGHSVVVIEQ 44 >S7335_385 FAD dependent oxidoreductase, putative Length = 438 Score = 29.3 bits (64), Expect = 0.71, Method: Compositional matrix adjust. Identities = 13/27 (48%), Positives = 18/27 (66%) Query: 6 VVGLGRSGSGAARLLQAQGYDVVVLER 32 ++G G SG +A LL QG++V V ER Sbjct: 5 IIGGGASGMASAYLLDQQGHEVTVFER 31 Database: GCA_000155595.1P Posted date: Aug 27, 2016 10:30 PM Number of letters in database: 1,647,188 Number of sequences in database: 5586 Lambda K H 0.319 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 5586 Number of Hits to DB: 1,557,000 Number of extensions: 66864 Number of successful extensions: 229 Number of sequences better than 1.0: 16 Number of HSP's gapped: 222 Number of HSP's successfully gapped: 16 Length of query: 462 Length of database: 1,647,188 Length adjustment: 88 Effective length of query: 374 Effective length of database: 1,155,620 Effective search space: 432201880 Effective search space used: 432201880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (26.9 bits)