BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= WH7805_12733 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
synthetase
(462 letters)
Database: GCA_000155555.1P
8294 sequences; 2,339,939 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MC7420_5843 UDP-N-acetylmuramoylalanine--D-glutamate ligase 340 e-112
MC7420_478 UDP-N-acetylmuramate--alanine ligase 58 1e-09
MC7420_1900 UDP-N-acetylmuramyl-tripeptide synthetases subfamily 50 3e-07
MC7420_2660 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine... 44 2e-05
MC7420_3428 FAD dependent oxidoreductase, putative 33 0.060
MC7420_430 FAD dependent oxidoreductase, putative 30 0.62
>MC7420_5843 UDP-N-acetylmuramoylalanine--D-glutamate ligase
Length = 465
Score = 340 bits (871), Expect = e-112, Method: Compositional matrix adjust.
Identities = 211/468 (45%), Positives = 273/468 (58%), Gaps = 23/468 (4%)
Query: 1 MSRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLGCPLEL 60
M + V+GLGRSG AARLL+ +G+ V V + +L+ + L + IT++LG L L
Sbjct: 1 MPNAHVIGLGRSGIAAARLLKQEGWTVTVSDSKTSESLQQQQQVLNVEGITVKLGYSLTL 60
Query: 61 -DSFTPWLDSAGEVVISPGIAWDHHTLHALRAHGFRVRGEMSVAWDALRGIPWIGITGTN 119
D P L +V+SPG+ WD L R G GEM +AW L+ PW+GITGTN
Sbjct: 61 GDGDLPEL-----IVVSPGVPWDIPVLIEARNRGIDTIGEMELAWRYLKSSPWVGITGTN 115
Query: 120 GKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTR-----------PKPDWVV 168
GKTT T L+ + AG AP GN+GY+A ELAL TR K DWV+
Sbjct: 116 GKTTTTALIAAIFQAAGFHAPACGNIGYAACELALAQPDKTRHGASLHEKLPQSKIDWVI 175
Query: 169 MEMSSYQIEAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDAD 228
E+SSYQIE++ E++P IG+WTT TPDHL RH T+E Y IK LL RS + N DD
Sbjct: 176 AEVSSYQIESSRELAPTIGVWTTFTPDHLNRHKTLENYYTIKADLLSRSHDQIFNGDDPY 235
Query: 229 IRQ-HCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVSRGEQHLFPADSLPLPGEHNR 287
+RQ HWS++ V D +P P + + GWV + + P DSL + G HN+
Sbjct: 236 LRQLGTTHWSKAYWTSVKGRDATPR---PWVYIE-NGWVMVESEPIVPVDSLRMVGNHNQ 291
Query: 288 QNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFNDSKATNYDAAAVG 347
QN+L+ AAA AG+ I A+ F GVPHRLE + + GI NDSKATNYDAA VG
Sbjct: 292 QNLLMAVAAARLAGIEKQAIVEAITNFPGVPHRLEHIITWQGIDFINDSKATNYDAAQVG 351
Query: 348 LRSVQAPVVLLAGGQTKKGDAVPWLKLIQSQVAGVVLFGEGAGELADLIETSGFAGPMIH 407
L +V +PV+L+AGG+ K GD WL IQ++ A V+L GE A A ++ G+ I
Sbjct: 352 LAAVDSPVILIAGGEAKAGDDTQWLNAIQAKAAAVLLIGEAASAFAQRLQEVGYFRYEI- 410
Query: 408 CADLDSAVSQALSLARNCQANSVLLSPACASFDQYPNFEARGDHFRQL 455
+ AV +A LA+ A VLLSPACASFD Y +FE RGD FRQL
Sbjct: 411 VETMARAVPRAAELAQQDNAPVVLLSPACASFDHYQSFEHRGDDFRQL 458
>MC7420_478 UDP-N-acetylmuramate--alanine ligase
Length = 512
Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust.
Identities = 57/226 (25%), Positives = 96/226 (42%), Gaps = 14/226 (6%)
Query: 113 IGITGTNGKTTVTHLLQHVLAHAGLEAPMAGNVGYSAAELALEVLQGTRPKPDWVVMEMS 172
I + GT+GKTT + L+ ++L GL+ + VG G P ++V E
Sbjct: 155 IAVAGTHGKTTTSSLMGYLLLKTGLDPTIV--VGGEVDAWQGNARLGKSP---FLVAEAD 209
Query: 173 SYQIEAAAEVSPRIGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEIPVLNADDADIRQH 232
+ A+++P IG+ T + DH + + ++ + +R + V D +R H
Sbjct: 210 ESD-GSLAKLAPEIGVITNIELDHPDHYQNLDQVIETFKIFADRCQTIVGCIDCQTLRSH 268
Query: 233 CGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVSR---GEQHLFPADSLPLPGEHNRQN 289
++ + D+S + ++ A+G +R G+Q + L L G+HN N
Sbjct: 269 FTPTISYSLQRTAGADYSVD----RIVYQAEGITARIWEGDQ-VLGTLQLSLLGQHNLSN 323
Query: 290 MLLVTAAALAAGVSASTIEAALRCFTGVPHRLEPLGSVGGIPVFND 335
L V A G+ I +AL F G R E G GI +D
Sbjct: 324 ALAVVAVGRHLGLEFDAIASALATFEGAKRRFEVRGECKGITFVDD 369
>MC7420_1900 UDP-N-acetylmuramyl-tripeptide synthetases subfamily
Length = 501
Score = 50.1 bits (118), Expect = 3e-07, Method: Compositional matrix adjust.
Identities = 56/219 (25%), Positives = 89/219 (40%), Gaps = 24/219 (10%)
Query: 126 HLLQHVLAHAGLEAPMAGNV-----GYSAA---------ELALEVLQGTRPKPDWVVMEM 171
HL++ +L A + G + G+S EL ++ + VME+
Sbjct: 131 HLIEFILTQAQRPTALFGTLYARWQGFSQTAVHTTPFPVELQQQLHSAVAAGCELGVMEV 190
Query: 172 SSYQIEAAAEVSPR--IGIWTTLTPDHLERHGTIEAYRAIKRGLLERSEI---PVLNADD 226
SS+ + + + ++T LT DHL+ H +E Y A K L + ++NADD
Sbjct: 191 SSHALAQGRVLGCEFDVAVFTNLTQDHLDFHRDMEDYFAAKALLFNSDYLHGKAIINADD 250
Query: 227 ADIRQHCGHWSRSCIRWVSCGDHSPEGGNPQLTVNAQGWVSRGEQHLFPAD---SLPLPG 283
++ R S + S + LT G +G H D + PL G
Sbjct: 251 PYGKRLIEKVGRDRTWSYSVQNPSADLYTSNLTYTLIG--VKGTLHTPVGDVTFNSPLVG 308
Query: 284 EHNRQNMLLVTAAALAAGVSASTIEAALRCFTGVPHRLE 322
++N N+L A L G+ T+ L F GVP R+E
Sbjct: 309 QYNLSNVLAAVGAVLNLGLDLKTVVTTLNQFPGVPGRME 347
>MC7420_2660 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
subfamily
Length = 466
Score = 44.3 bits (103), Expect = 2e-05, Method: Compositional matrix adjust.
Identities = 57/207 (27%), Positives = 87/207 (42%), Gaps = 30/207 (14%)
Query: 104 WDALRGIPWIGITGTNGKTTVTHLLQHVLAHAG----LEAPMAGNVGYSAAELALEVLQG 159
W IP I +TG+ GKTT L+ VL+ G +A +G L L+
Sbjct: 103 WRDQFDIPVIAVTGSAGKTTTKELIAAVLSTQGNILKTQANYNNEIGVPKTLLELD---- 158
Query: 160 TRPKPDWVVMEMS---SYQIEAAAEVS-PRIGIWTTLTPDHLERHGTIEAYRAIKRGLLE 215
D+ V+EM+ S QI +++ P IG+ T + H+ G+ +A K LL
Sbjct: 159 --SNHDYAVIEMAMRGSGQIALLTQIARPTIGVITNVGTAHIGLLGSEDAIAKAKCELLA 216
Query: 216 R---SEIPVLNADDADIRQHCGH-WSRSCIRWVSCGDHSPEGGNPQ-LTVNAQGWVSRGE 270
+ + + +LN D+ + W + + GG+ Q ++ + G
Sbjct: 217 QMPDTSMAILNHDNQRLMDTAATVWQGETLTF------GLNGGDVQGKLIDLFRLMVDGI 270
Query: 271 QHLFPADSLPLPGEHNRQNMLLVTAAA 297
FP LPLPGEHN N L A A
Sbjct: 271 T--FP---LPLPGEHNALNYLAALAVA 292
>MC7420_3428 FAD dependent oxidoreductase, putative
Length = 503
Score = 33.5 bits (75), Expect = 0.060, Method: Compositional matrix adjust.
Identities = 18/35 (51%), Positives = 20/35 (57%)
Query: 2 SRSVVVGLGRSGSGAARLLQAQGYDVVVLERGDEP 36
R VVVG G +G GA L QGYDV +LE P
Sbjct: 8 KRIVVVGAGWAGLGATYHLAQQGYDVTLLEAAPYP 42
>MC7420_430 FAD dependent oxidoreductase, putative
Length = 494
Score = 30.0 bits (66), Expect = 0.62, Method: Compositional matrix adjust.
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Query: 5 VVVGLGRSGSGAARLLQAQGYDVVVLERGDEPALRDKAHALRQQNITIQLG 55
VV+G G G AA LQA G+ V ++E+ D R A+ +QQ T G
Sbjct: 4 VVIGSGFGGLSAAIRLQAGGHRVTIVEKRDRAGGR--AYVYQQQGFTFDGG 52
Database: GCA_000155555.1P
Posted date: Aug 27, 2016 10:38 PM
Number of letters in database: 2,339,939
Number of sequences in database: 8294
Lambda K H
0.319 0.134 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 8294
Number of Hits to DB: 2,221,309
Number of extensions: 95111
Number of successful extensions: 278
Number of sequences better than 1.0: 13
Number of HSP's gapped: 270
Number of HSP's successfully gapped: 13
Length of query: 462
Length of database: 2,339,939
Length adjustment: 90
Effective length of query: 372
Effective length of database: 1,593,479
Effective search space: 592774188
Effective search space used: 592774188
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)